Isolation and amplification DNA of Elaeis guineensis from Bangun Bandar Plantation, North Sumatra

Abstract

Elaeis guineensis is the primary commodity that supports the economy of Indonesian country. Lately, several reported diseases of oil palm caused by fungi, have declined in the quality of oil palm plantation also caused the death of several mature oil palm plants. The disease damages roots or tree trunk tissue is known as Basal Stem Rot (BSR). The present study aimed to report explains of the PCR and UV-tex method for detecting Ganoderma in mature palm plants. PCR can detect disease and primer EgMT used to produce various sizes of PCR products. Each sample has different brightness amplification depending on the reaction of the plant to the BSR fungus. The primer is expected to be suitable for detecting Ganoderma fungus in dura, pisifera and tenera oil palm mature plants. The length of the DNA fragment is visible with UV-1D after being irradiated with UV light. DNA amplification ranged from a value of 10,526-114,289 bp. The type of dura plant detected by amplified BSR disease was 34,211-114,289 bp. On the other hand, pisifera is only 21,053 bp and tenera 10,526-104,844 bp.