PREPARATION OF LYSOZYME ENZYME COATED WITH CHITOSAN NANOPARTICLES

Abstract

In this study, the lysozyme enzyme was encapsulated with chitosan nanoparticles by adopting the ionic gelation technique, where samples were prepared from different concentrations of lysozyme and chitosan nanoparticles. With the presence of triphosphate polyphosphate (TPP). Lys-CS-NPs particle sizes ranged between 30-105 nm for the treatment A3 and a rate of 68.7 nm and for the A4 treatment between 30-90 nanometers and a rate of 67.79 nm, meaning that they were within the nanometer, and their Zeta potential reached +50.41 and +47.55 mV for the two treatments respectively. That is, it was of good stability. SEM microscope images showed that the liquid particles were of uniform swollen spherical shapes for the two parameters and their sizes were larger than the freeze particles, which had a solid structure and a spherical, orderly and symmetrical shape, but the particles in treatment A3 were more organized and coordinated than the treatment A4. The AFM images showed the surface shape of the samples for the two parameters A3 and A4, which were divided into three regions according to color, a region with white spherical shapes representing chitosan particles, and a small white point area representing chitosan nanoparticles, while the third region appeared in the dark golden brown color and in small, inconsistent shapes. Size They represent Lys-CS-NPs .The enzymatic activity of Lys-CS-NPs was assessed to see if the chitosan-coated lysozyme still retained its efficacy after release by comparing its enzymatic activity with the activity of the non-coated enzyme, after keeping the enzymes by cooling for 48 hours to allow it to be released from particles showed high efficacy. Compared with uncoated lysozymes, treatment A3 reached 1.657 units / ml, and for treatment A4 1.480 units / ml, while the activity of the uncoated enzyme was 1.159 units / ml.