The effect of buffers on weak acid uptake by vesicles

Abstract

The assessment of weak acid membrane permeability (P m ) frequently involves large unilamellar vesicles. It relies on measurements of the intravesicular pH drop, ΔpH in , in response to a sudden augmentation of external acid concentration. However, ΔpH in may be primarily governed by non-instantaneous protonation and deprotonation reactions of (i) the acid itself, (ii) the buffer molecules, and (iii) the fluorescent pH reporter dye. Moreover, buffer concentration and acid gradient also serve as determinants of ΔpH in , as we show here. The uniexponential time constant (τ) of ΔpH in (t) is an invalid measure of P m as Arrhenius plots of P m and reveal different activation energies for acid influx. We calculate P m by fitting a mathematical model to experimental stopped-flow traces. The model takes into account not only the time course of total internal buffer capacity but also (i) water self-dissociation, (ii) volume changes due to acid induced osmotic water flow, and (iii) the spontaneous membrane proton leak. It allows extracting a P m of 30.8 ± 3.5 µm⁄s for formic acid for 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) vesicles.