Genetic Identification of POU1F1|PstI gene of Lakor Goat from Lakor Island, Southwest Maluku Regency

Abstract

Lakor goat is a domestic animal from Lakor island in South West Maluku Regency. They successfully survive and reproduce with limited water, grass and high temperature. Growth traits is an economic trait which important for breeding strategy (productivity improvement). Pituitary Specific Transcription Factor 1 (POU1F1) is otherwise known as PIT1 gene plays a role of growth and carcass traits. The objective of this study was to identify the POU1F1 gene in Lakor goat from Lakor island in Southwest Maluku Regency. Sixty-Three samples of hair tail were collected from three different locations: Ketti Letpey (18), Werwawan Jamluli (26) and Letoda (19). DNA was extracted by DNA isolation kit. POU1FI gene was amplified using PCR technique while genotyping used PCR-RFLP with PstI restriction enzyme. Result showed that only one genotype (TT) was found and monomorphic. It may be caused remote area and limited of ram that indicated inbreeding has occurred. Inbreeding rate or coefficient study and introgression of ram from out of Lakor island by Artificial Insemination (AI) or natural mating is needed.