Some properties of β-1,2-mannosyltransferases related to the biosynthesis of the acid-labile oligomannosyl side chains in Candida albicans NIH B-792 strain cells

Abstract

We detected the β-1,2-mannosyltransferases (β-1,2-MTs), which participate in the biosynthesis of oligomannosyl side chains in the mannan acid-labile fraction, in a particulate insoluble fractions prepared from Candida albicans NIH B-792 strain cells grown at 27°C and at 37°C in a yeast extract-added Sabouraud liquid medium (YSLM). The β-1,2-MT VI-6 prepared from the cells grown at 27°C exhibited the maximum activity at pH 7.0 and at 30°C. The β-1,2-MT VI-6 activity was only slightly affected by Mn 2+, Mg2+, Ca2+, and ethylenediamine-tetraacetic acid, but completely inhibited by Zn2+ and Ni2+. The β-1,2-MT activities from the cells grown at 37°C were lower than that from the cells grown at 27°C, especially on the longer β-1,2- mannooligosaccharides than tetraose. © 2009 Pharmaceutical Society of Japan.