Standardization of Prasaranyadi Kashayam – a classical Ayurvedic polyherbal formulation

Abstract

Prasarinyadi Kashayam is used to treat Vata illnesses and joint discomfort in the Ayurvedic system of medicine. It is mostly used to alleviate musculoskeletal issues and joint pain. It has traditionally been used to treat cervical spondylosis, frozen shoulders, headaches, lower back pains, and stiffness related to lumbar spondylosis. The present work was aimed at developing the pharmacognostic parameters for the standardization and quality evaluation of the Prasaranyadi Kashayam. In-house polyherbal formulation was prepared as per the previously prescribed method, and physicochemical parameters were evaluated as per standard protocol and showed loss on drying of 99.31%, total ash of 4.911 ± 0.120%, water soluble ash of 3.022, acid insoluble ash of 0.489. Total phenolic content was estimated with FC reagent and expressed as gallic acid equivalent (9.95 + 0.004 mg/g). High performance thin layer chromatography (HPTLC) fingerprint profiling was obtained, using toluene, ethyl acetate, and formic acid (2.5, 2.0, and 0.5) as the mobile phase. The characterization parameters presented in this paper may serve as a standard reference for quality control analysis of Prasaranyadi Kashayam.