Enhancement of saponin accumulation in adventitious root culture of Javanese ginseng (Talinum paniculatum Gaertn.) through methyl jasmonate and salicylic acid elicitation

Abstract

Javanese ginseng (Talinum paniculatum) is an herb and one of the traditional medicinal plants in Indonesia which accumulate saponin in the root organ. However, slow root growth hampers the accumulation of the compd. in this plant. Therefore, in vitro culture of adventitious roots offers an alternative way to increase the prodn. of saponin. Furthermore, this study was performed to investigate the effect of Me jasmonate (MeJA) and salicylic acid (SA) on the growth and saponin content in adventitious root of Javanese ginseng. Adventitious root culture was induced on solid Murashige and Skoog (MS) medium supplemented by 10 μM IBA (indole-3-butyric acid). Subsequently, adventitious roots were subcultured into a half strength MS liq. medium with the addn. of different concns. of MeJA or SA and incubated for 5, 10 and 15 days. Despite inhibition on the root growth, saponin prodn. increased by 1.5 and 1.3 fold upon elicitation with 0.2 mM MeJA and SA for 15 days, resp. Thus, MeJA and SA elicitations regulate saponin biosynthesis in adventitious root culture of Javanese ginseng as a time and dose-dependent manner. [on SciFinder(R)]