Study on a Saliva Test for Periodontal Disease in the Elderly.

Abstract

We studied risk indicators in saliva for periodontal disease in the elderly. Thirty-six subjects over 65 years old (15 men and 21 women) were divided into 4 groups based on clinical evaluation: 7 with healthy gingiva or gingivitis, 11 with moderate periodontitis, 9 with severe periodontitis, and 9 edentulous subjects. Whole saliva samples were analyzed for the presence of Actinobacillus actinomycetemcomitans, Porphyro-monas gingivalis, Prevotella intermedia, Bacteroides forsythus, and Treponema denticola using polymerase chain reaction (PCR) method. We studied the relationship of periodontal parameters to the presence of these periodontopathic bacteria, lactate dehydrogenase (LDH) , aspartate aminotransferase (AST) , and interleukin-1 (IL-1) gene polymorphism. The prevalence of Bacteroides forsythus in subjects with severe periodontitis was significantly higher than in those with healthy gingiva or gingivitis (p<0.05). LDH activity increased proportionally with the advance of periodontitis (p<0.05). AST and IL-1 polymor-phism did not correlate significantly with periodontal status. This suggests that the presence of Bacteroides forsythus and LDH activity in whole saliva are possible to be risk indicators for periodontal disease in the elderly.-v Ž|:-{ OE¤ ‹ † ‚Ì-Ú"I ‚Í, •‚-î ŽÒ ‚Ì Ž• Žü •a ‚ð•f 'f ‚· ‚é'Á ‰t ' † ‚̈ö‚̈ö Žq ‚É ‚ ‚¢ ‚ÄOEŸ "¢ ‚· ‚é ‚± ‚AE‚Å ‚ ‚é •B65•Î ˆÈ •ã ‚Å, OE' •í OEQ7-¼, Ž• Žü ‰Š ‚Ì ' † "™ "x OEQ11-¼, •d "x OEQ9-¼, ‚Ü‚½-³ Ž• Š{ OEQ9-¼ ‚Ì OEv36-¼ ‚ð"í OE± ŽÒ ‚AE ‚µ‚½ •B 'S 'Á ‰t ‚ð-p ‚¢ ‚Ä, Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Bacte-roides forsythus, Treponem ‚ðPolym crascchain rcaction-@ (PCR-@) ‚É ‚Ä OEŸ •o, Lactatc dehydrogenase (LDH) ‚âAspartateaminotransferase (AST) ‚Ì Šˆ•Šˆ•« 'l 'ª 'è, ‚¨‚ae‚¨‚ae ‚Ñ ƒC ƒ" ƒ^ •[ ƒ• ƒC ƒL ƒ" 1 (IL-1) ‚̈â "` Žq '½ OE^ ‚ðOEŸ •õ ‚µ, Še OEQ ‚Ì"ä Šr, ‚¨‚ae‚¨‚ae ‚Ñ-Õ •° ƒp ƒ‰ ƒ• •[ ƒ^ •[ ‚AE‚Ì ŠÖ˜AŠÖ˜A ‚ð'² ‚× ‚½ •B ‚» ‚Ì OE‹ ‰Ê, Bacte-roidesforsythus‚Ì OEŸ •o-¦ ‚ª •d "x OEQ ‚ÅOE' •íOEQ ‚ae ‚è-L ˆÓ ‚É•‚ ‚© ‚Á ‚½ (P<0.05) •B ‚Ü ‚½, LDHŠˆ•LDHŠˆ•« ‚ÍŽ• Žü •a ‚ª•d "x ‚É ‚È ‚é ‚Ù ‚Ç•‚ ‚-‚È ‚Á ‚½ (P<0.05) •BASTŠˆ• BASTŠˆ•« ‚AEIL-1‚̈â1‚̈â "` Žq '½ OE^ ‚É ‚¨‚¢‚¨‚¢ ‚Ä ‚Í, Ž• Žü •a ‚AE ‚Ì-¾ ‚ç‚© ‚È ŠÖ˜AŠÖ˜A ‚ª "F ‚ß ‚ç‚ê ‚È ‚© ‚Á ‚½•B ‚ae ‚Á‚Ä, 'Á ‰t ‚© ‚çOEŸ •o ‚³ ‚ê ‚é •BBacteroides forsythus‚Ì-L-³ ‚AELDH‚Ì 'l ‚Í, •‚-î ŽÒ ‚Ì Ž• Žü •a •f 'f ‚É-L-p ‚Å ‚ ‚é ‚± ‚AE‚ª Ž¦ •´ ‚³ ‚ê ‚½ •B •õ ˆø-p OEê: •‚-î ŽÒ, 'Á ‰t, PCR, LDH