Comparaison des ectomycorhizes naturelles entre le hetre (Fagus sylvatica) et deux lactaires (Lactarius blennius var viridis et Lactarius subdulcis). II. Caracterisation cytochimique des interfaces

Abstract

Fagus sylvatica/Lactarius blennius var viridis and Lactarius subdulcis ectomycorrhizae were studied cytochemically (PATAg test, Gomori-Swift reaction, WGA-colloidal gold labelling) to characterize cell wall and matrix components. Different intensities of reaction were observed between walls of both Lactarius species and according to interfaces in the one mycorrhiza. The mantle-soil interface of L blennius mycorrhizae was characterized by mucilagenous material rich in chitin. L subdulcis mycorrhizae were infected by an ascomycete whose walls were bordered by an electron-dense layer. A decrease of chitin content was observed in the ascomycete walls between the hyphae present in the mantle and intracellularly. In both mycorrhizae, the PATAg test revealed an accumulation of glycogen in rosettes along the plasmalemma of the Hartig net hyphae.