PEMANFAATAN DARAH SISA TRANSFUSI DALAM PEMBUATAN MEDIA BAP UNTUK PERTUMBUHAN BAKTERI Streptococcus pyogenes

Abstract

Streptococcus pyogenes bacteria causes infectious diseases in the respiratory tract. Gold standard for the diagnosis of diseases caused by Streptococcus pyogenes is culture. The special media used is blood agar media. Blood agar media contains special nutrients for bacterial growth, which are enriched with animal or human blood. In Indonesia, wool sheep blood is not easy to obtain, because it is difficult to breed and cannot live adapt to the tropical climate. The alternative is to use human blood as an essential compound for making blood agar media (Abdat, 2010). Based on interviews with several nurses in the hospital there is a phenomenon of blood loss caused by transfusion reactions in patients, so that blood cannot be used anymore. The existence of this phenomenon researchers are interested in conducting research on the utilization of transfusion residual blood that is used as raw material for the manufacture of BAP media in isolation of the growth of the bacteria Streptococcus pyogenes. The number of Streptococcus pyogenes bacterial colonies growing in the media so that sheep blood reaches 170 while in the media so that humans only reach 143 and more colonies that grow in the media so that sheep blood because the blood used is still fresh, so the blood component is still intact, like hemoglobin and erythrocytes haven't died yet