Lipases and proteases produced by indigenous Pseudomonas aeruginosa strain as potential detergent additives

Abstract

Enzymes produced by indigenous Pseudomonas aeruginosa strain have been subjected to research considering their potential application as detergent additives. As previously noted, lipase produced by Pseudomonas aeruginosa is highly alkaline, thermostable and solvent tolerant. Furthermore, same strain exhibits both lipase and protease activity establishing this lipase as potentially desirable component of enzyme-containing detergents. Further research was carried out to investigate insusceptibility of this lipase against coexisting native protease, several commercial surfactants, oxidizing agents and commercial detergents. Lipases and proteases remained highly active when incubated with several different surfactants and oxidizing agents under washing conditions. Moreover, presence of surfactants and oxidizing agents such as Tween? 20 and Triton? X-100 initially augment lipase and protease activity. Additionally, crude lipase preparation was insusceptible to coexisting native protease hence indicating possible storage stability. Overall, the remarkable properties of these enzymes make them potential detergent additives.Enzimi iz ekstremofilne Pseudomonas aeruginosa vrste pokazuju visoku stabilnost u alkalnoj sredini sto ih cini pogodnim za primenu u proizvodnji deterdzenata. U ovom radu ispitana je aktivnost lipaza i proteaza dobijenih iz ove vrste u uslovima od znacaja za industriju deterdzenata. Ustanovljeno je da u realnim uslovima pranja, oba enzima zadrzavaju visoku aktivnost u prisustvu razlicitih povrsinski aktivnih materija i oksidacionih agenasa. Takodje, prisustvo nekih aditiva kao sto su Tween? 20, Triton? X-100, SDS i NaClO u pocetnoj fazi povecava lipoliticku aktivnost dok je povecanje proteoliticke aktivnosti primeceno u formulacijama koje sadrze Tween? 20 i Triton? X-100. Nadalje, sama lipaza je ocuvala aktivnost u prisustvu nativnih proteaza sto upucuje na zakljucak da bi ovi enzimi zadrzali visoku efikasnost u formulacijama deterdzenata i tokom procesa pranja/ciscenja.