Geographic differentiation and embryo type identification in Mangifera indica L. cultivars using RAPD markers

Abstract

Fifteen mango cultivars were examined using randomly amplified polymorphic DNA (RAPD) markers with decamer primers of arbitrary sequence. Thirteen of the 40 primers screened were informative and 109 amplified DNA bands were selected as RAPD markers. Specific RAPD markers for some mango cultivars were identified. Cluster analysis based on the 109 RAPD markers produced a dendrogram of the genetic relatedness of the 15 mango cultivars. 'Manila' and 'Carabao' were the most similar, which is in good agreement with their putative pedigrees. The four major bifurcations in the dendrogram separated correctly the genotypes into four groups according to their geographic origin. Bulk segregant analysis of polyembryonic and monoembryonic cultivars detected a specific RAPD marker for polyembryony. These markers may facilitate the management of mango germplasm for breeding purposes.