Assessment of anti bacterial, anti inflammation and wound healing activity in Wistar albino rats using green silver nanoparticles synthesized from Tagetes erecta leaves

Abstract

Silver nanoparticles synthesized from plant material have superior bioactivities. The purpose of this current study was to synthe-sis, characterize and to explore the bioactive efficacy of silver nanoparticles (Ag-NPs) using aqueous leaf extract of Taget-eserecta. The biosynthesized Ag-NPs were characterized using ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction and Scanning electron microscopy. Ag-NPs were studied for in-vivo anti-inflammatory and wound healing activities performed in female Wistar albino rats. UV -Vis absorption spectrum of the T.erecta leaves extract was obtained at 428nm due to excitation of surface plasmon vibration in nanoparticles and confirms the synthesis of silver na-noparticles. The average crystallite size of AgNP synthesized was found to be 27.2 nm. The spherical silver grain of 15.5 nm average size has been depicted with high-resolution scanning electron microscopy. Maximum activity (15mm) of T.erecta leaves silver nanoparti-cles was observed against Salmonella typhi (15mm) followed by Escherichia coli (12mm). Ag-NPs exhibited significant wound healing activity and anti-inflammatory activity in carrageenan-induced paw volume tests performed in female Wistar albino rats. Colloidal Ag-NPs can be synthesized by simple, nonhazardous methods, and biosynthesized Ag-NPs using T.erectaleaves extract have significant therapeutic properties.This work evidently confirmed that silver nanoparticles mediated T.erecta could be considered as a potential source for anti-inflammatory and wound healing drug.