The effect of growth regulators on the regeneration ability of flax {Linum usitatissimum L.) hypocotyl explants in in vitro culture

Abstract

The aim of the present study is to determine the influence of different concentrations of growth regulators on the hypocotyl explants regeneration abilities of flax cultivars (Linum usitatissimum L.). Plant material used in the study was obtained from two flax cultivars: Modran and Selena. Hypocotyl explants were obtained from 6-day-old seedlings and were subsequently placed on basal MS (Murashige and Skoog 1962) medium and MS medium supplemented with 6-benzylaminopurine (BAP) or naftalene acetic acid (NAA). Final evaluation of the callus, the shoot and the root formation of the explants was performed 28 days after the experimental setup. The highest regeneration effectiveness was observed on the media supplemented with 1 mg/1 BAP.