PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT ACTIVITY OF METHANOL EXTRACT OF ZANTHOXYLUM ACANTHOPODIUM DC. FRUITS USING CUPRAC METHODS

Abstract

Objective: Free radicals are substances that can be harmful to our bodies if their concentrations are too high. Free radicals are neutralized by antioxidant molecules, which additionally protect cells against damage from oxidation caused by free radicals. This study aims to conduct a phytochemical analysis and quantify the antioxidant capabilities of the methanol extract of Zanthoxylum acanthopodium DC. fruits. Methods: Zanthoxylum acanthopodium DC. fruit was extracted by reflux method using methanol. Antioxidant activity for extract was measured with CUPRAC (Cupric Ion Reducing Antioxidant Capacity) method and LC-HRMS (Liquid Chromatography High Resolution Mass Spectrometry) instrument was used for phytochemical analysis of methanol extract. Results: The IC50 of methanol extract was 86.58±1.87 μg/ml. LC-HRMS analysis showed that there were 10 active compounds detected in methanol extract of Zanthoxylum acanthopodium DC. The content of secondary metabolite compound was group of flavonoid (“neohesperidin, isorhamnetin, astragalin, quercetin, quercetin-3β-D-glucoside, (2E)-4-Hydroxy-3,7-dimethyl-2,6-octadien-1-yl beta-D-glucopyranoside, and 5,7-Dihydroxy-2-(4-hydroxyphenyl)-6,8-bis[3,4,5-trihydroxy-6-(hydroxymethyl) tetrahydro-2H-pyran-2-yl]-4H-chromen-4-one”) and phenolic (Chlorogenic acid, quinic acid). Conclusion: The results show that the methanol extract from the fruit of Zanthoxylum acanthopodium DC. has potent antioxidant properties and contains several flavonoid and phenolic components.