Physiological relationship between content of certain microelements in the tissues of different anatomic sections of the organism of honey bees exposed to citrates of argentum and cuprum

Abstract

The study was conducted on honey bees of the Carpathian breed on the basis of the apiary of S. Z. Gzhytskyi Lviv National University of Veterinary Medicine and Biotechnologies. The bees were kept in 8-frame beehives with size of the frame of 435 × 300 mm. The study was undertaken in the summer-autumn period. A total of 5 groups of bee families were formed according to the analogue principle, with three families of bees in each group. Control (I) group received sugar syrup in 1: 1 concentration (1000 mL/group/week). In the experimental (II) group, Ag was added to the syrup in amount of 0.5 mL/g of the sugar syrup; in III experimental group – in similar conditions Ag was added in 1 mg/L amount; IV group received sugar syrup with Cu in 0.5 mg/L amount; V experimental group – Cu in amount of 1 mg/L of sugar syrup. Microelements were added to sugar syrup in the form of citrates obtained using method of nanotechnology, developed by the company Nanomaterials and Nanotechnologies in Kyiv. The period of monitoring lasted 28 days with 7 days interval of preparation. Inter-group and anatomic differences were found in content of certain mineral elements in the tissues of the head, thorax and abdominal sections of the honey bees in conditions of feeding with citrates of argentum and cuprum in sugar syrups. Under the influence of citrate of argentum in amount of 0.5 and 1.0 mg/L of sugar syrup, content of ferrum increased in the tissues of the head and abdominal sections, though it decreased in the thorax, content of cuprum decreased in the tissues of head, thorax and abdomen, content of zinc increased in the tissues of head and thorax, though it decreased in the abdomen, content of lead and cadmium reduced in the tissues of all anatomic sections of the organism of honey bees. Under the influence of cuprum in amount of 0.5 and 1.0 mg/L of sugar syrup, content of ferrum reduced in the tissues of the head, though it increased in the tissues of thorax and abdomen, content of cuprum increased, and the content of zinc, lead and cadmium reduced in the tissues of all anatomic sections of honey bees. According to the results of the study, the determining effect of feeding of honey bees on the optimization of the parameters of mineral metabolism in different anatomic sections of the organism of bees is discussed.