A Chemiluminescence Method for Determination of Lipid Hydroperoxides

Abstract

A chemiluminescence method is described for the determination of lipid hydroperoxides in chloroform-methanol extracts of foods or biological materials. The method is based on registration of the luminol-dependent light emission induced by hemoglobin-catalyzed decomposition of hydroperoxides. The applicability of the assay and the optimal conditions for assessment of lipid hydroperoxides in different systems were determined. The main advantages of the chemiluminescence method are its high specificity and sensitivity. By this method 0.01 nmol of lipid hydroperoxides could be determined. © 1990, SOCIETY FOR FREE RADICAL RESEARCH JAPAN. All rights reserved.