Establishment of a defined minimal medium and isolation of auxotrophic mutants for bifidobacterium bifidum ES 5

Abstract

A perfectly defined minimal medium for strain Es 5 of Bifidobacterium bifidum was established. It consists of 3.5 % D-(+)-lactose, 2.5 % sodium acetate, 0.2 % ammonium acetate, 0.25 % dipotassium phosphate, 50 ug/ ml riboflavin, 50 ug/m1 D-pantethine, 50 ng/ml biotin, 0.3% potassium pyruvate, 0.1% L-cysteine-HCl, 0.5 mM MgCL2, ImM FeCl2, 0.5 uM MnCl2 and 50 uM CaCl2 (pH 6.6). Many auxotrophic mutants were isolated from the strain using this minimal. © 1983, Applied Microbiology, Molecular and Cellular Biosciences Research Foundation. All rights reserved.