A novel stress-acclimation response in Spirodela punctata (Lemnaceae): 2,4,6-Trichlorophenol triggers an increase in the level of an extracellular peroxidase, capable of the oxidative dechlorination of this xenobiotic pollutant

Abstract

Peroxidases are haem-containing enzymes capable of oxidizing a wide range of substrates. This article describes the presence of peroxidase activity in the growth medium of axenic Spirodela punctata (Lemnaceae) cultures. It was found that the release of extracellular peroxidase activity is specifically enhanced by phytotoxic, halogenated phenols but not by other abiotic stress-factors, elicitors or plant metabolites. Based on the concentration dependence of 2,4,6-trichlorophenol (TCP)-enhanced peroxidase release, it is concluded that release is not simply a consequence of physiological damage, but rather requires metabolically healthy fronds. In vitro studies (UV/VIS spectroscopy and liquid chromatography/mass spectrometry) show that the extracellular duckweed peroxidase (SpEx), which was partially purified from Spirodela growth medium, is capable of catalysing the oxidative dechlorination of TCP with hydrogen peroxide as the electron acceptor. It is proposed that the ability of S. punctata to specifically sense environmentally persistent phytotoxic chlorophenols, and to respond by increasing extracellular levels of a peroxidase capable of catalysing their oxidative dechlorination, is part of the protection strategy of this aquatic plant against xenobiotic stress.