Quantitative Methods for Alkaline Protease Determination and its Applications: A Comprehensive Review

Abstract

Proteases break peptide bonds. It is often necessary to measure and/or compare the activity of alkaline proteases using different procedures in the lab. Many studies with keen interest on alkaline proteases mostly use quantitative and/or qualitative assay methods to assay the enzyme activity of proteases. There is need to select a suitable assay method from the reported ones which will be ideal for any proposed study. There could be challenges when choosing the right assay method from the existing ones, thereby prompting the need for a review of the various methods for the quantitative assay of alkaline protease, the quantitative methods and protocols used from 1938 until now and their industrial applications were chronologically reviewed.