Research on extraction and characterization of cellulase from commercial enzyme preparation

Abstract

The extraction of cellulase from commercial enzyme preparation by ammonium sulfate precipitation and its enzymatic characterization were studied. The results were as follows: the conditions of precipitation was 60% saturation of ammonium sulfate, the recovery rate of carboxymethylcellulase (CMCase) and Filter Paper enzyme Activity (FPA) were 73.8 and 71.4%, respectively. The enzyme has optimal temperature of 55°C, optimal pH of 4.8, the ions of Co2+, Mn2+ in the buffer lowered the activity of CMCase, but the Cu2+ in low concentration activated the CMCase. The ions of Co2+, Mn2+ and Al3+ in the buffer lowered the activity of FPA, but the Cu2+and K+ in low concentration activated the FPA; Km and Vmax of the enzyme were 2.22×10-3g/mL and 1.11×104 U/h using Lineweaver-Burk graphic method, respectively. © Maxwell Scientific Organization, 2013.