Separation and Determination of cis-β-Carotene and trans-β-Carotene by High Performance Liquid Chromatography

Abstract

A high-performance liquid chromatographic method using a general ODS column (YMC PACK ODS-A) was established for separation and determination of cis-β-carotene, trans-β-carotene and α-carotene. cis-β-Carotene, trans-β-carotene and α-carotene were separated on an ODS column (5 μm, 4. 6 X 250 mm) with a mobile phase that consisted of methyl alcohol-acetonitrile-n-hexane-ethyl ether (15: 70: 10: 5). The flow rate was 1. 0 ml/min, and the detection wavelength was 453 nm. Then a-carotene and trans-β-carotene contents were calculated according to calibration curves obtained by the internal standard (β-apo-8'-carotenal) method. Total carotene was determined with a mobile phase that consisted of benzene-n-hexane (1: 5). © 1990, Japanese Society for Food Hygiene and Safety. All rights reserved.