Interdigitated Microelectrode Arrays Integrated in Microfluidic Cell for Biosensor Applications

Abstract

In this work we present an approach for the use of phages as bioreceptors for bacteria detection in a microfluidic cell by Electrochemical Impedance Spectroscopy (EIS). With this aim, the biocomponent has been immobilized on interdigitated gold microelectrodes with the phyisorption technique. The three electrodes (reference electrode, counter electrode and work electrode) are integrated on the same chip and a microfluidic cell is used. We have shown that chip is stable in liquid medium for several hours. For the detection side, the interaction of E.coli bacteria with T4 phages shows an increase of the impedance. A detection limit of 103 CFU mL-1 was obtained with good reproducibility