Phytochemical Content and Antioxidant Activity in Wild Grape (Ampelocissus martini Planch.) Root Extracts

Abstract

The factionated extracts of wild grape (Ampelocissus martini Planch.) roots by silica gel chromatography eluting with different solvent systems were firstly prepared, and then were assayed for their phytochemical contents and antioxidant activity. A high level of total phenolic and flavonoid content were found in both crude extract and chromatographic fractions. The ethyl acetate: methanol (75:25 %v/v) fraction had the highest of total phenolic and flavonoid as well as antioxidant activity assessed all methods, except ABTS assay. This indicated that eluting solvents directly affected phytochemical profile and activity. Moreover, the phenolic and flanonoid substances showed highly possitive correlation coefficient (r) to antioxidant activity. This suggested that the wild grape root is a natural source containing high phytochemicals with antioxidant activity which might be used as active ingredient for health supporting.