Reference materials for optic nanosensor systems: reduced glutathione and chloramphenicol

Abstract

The research provides enterprise reference material (ERM) development approach for nanosensor system on the basis of dynamic light scattering and fluorescence for qualitative and quantitative determination of nutrition pollutant - chloramphenicol antibiotic (laevomycetin), as well as marker of ischaemic stroke and a number of other diseases - reduced glutathione. ERM candidate materials were chosen chloramphenicol and reduced glutathione. The attestation procedure based on calculating-experimental approach by preparation procedure was used to determine the certified value of ERM. During the tests there were determined metrological characteristics of reference material. The certified value of ERM for reduced glutathione mass fraction is 98,5 %, the expanded uncertainty of certified value with coverage factor k=2 is ± 0,3 %. The certified value of ERM for chloramphenicol mass concentration is 10,0 g/dm 3 , the expanded uncertainty of certified value with coverage factor k = 2 is ± 4,0 %. The implementation of the developed ERMs showed their appropriateness for optic nanosensor systems calibration on the base of dynamic light scattering and fluorescence.We suppose that the developed enterprise reference materials further may be certified as CRM (certified reference material) and used for verification, calibration and graduation of compact detection devices of the «point of care diagnostics» type under development, which are intended for express analysis on the sampling site directly.