Gateway binary vectors with the bialaphos resistance gene, bar, as a selection marker for plant transformation

Abstract

We constructed two series of Gateway binary vectors, pGWBs and R4pGWBs, possessing the bialaphos resistance gene (bar) as a selection marker for plant transformation. The reporters and tags employed in this system are sGFP, GUS, LUC, EYFP, ECFP, G3GFP, mRFP, TagRFP, 6xHls, FLAG, 3xHA, 4xMyc, lOxMyc, GST, 17 and TAP. Selection or ArabidopsLs transform ants with BASTA® was successfully carried out using both plate-grown and soil-grown seedlings. Transformed rice calli and suspension-cultured tobacco cells were selected on plates containing BAST A® or glufosinate-ammonium. These vectors are compatible with existing pGWB and R4pGWB vectors carrying kanamycin and hygromycin B resistance.