Chromosome variation in callus and regenerants of Podophyllum hexandrum (Podophyllaceae)

Abstract

The cytological status of callus and regenerants of Podophyllum hexandrum cultured in vitro for over a period of 3 years is reported. Calli maintained both on lower (0.5 μM) and higher (10 μM) 2, 4-D, remained cytologically stable up to 18 months. With the long term (3 years) culture on higher 2, 4-D both numerical and structural changes in the chromosomes were observed. The calli maintained for 3 years on lower 2, 4-D on the other hand retained both chromosome stability and regeneration potentiality. The regenerants in general showed normal mitotic complements. One of the regenerants from higher 2, 4-D medium, however, showed structural chromosome changes. © 1994 Taylor & Francis Group, LLC.