Polymerase chain reaction protocols for alpha globin haplotype polymorphisms

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Abstract

Polymerase chain reaction protocols were designed specifically to amplify regions of the alpha globin complex that contain the nine common polymorphic haplotyping sites. These reactions provided a quicker and more sensitive approach to determining alpha globin haplotypes than Southern blotting methods.

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Miles, K., Norwich, J. T., Martinson, J. J., & Clegg, J. B. (2001). Polymerase chain reaction protocols for alpha globin haplotype polymorphisms. British Journal of Haematology, 113(3), 694–698. https://doi.org/10.1046/j.1365-2141.2001.02823.x

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