Identification of metabolites of tectoridin in-vivo and in-vitro by liquid chromatography-tandem mass spectrometry

Abstract

In this work, liquid chromatography-electrospray ionization tandem ion-trap mass spectrometry (LC-MSn) was used to investigate the in-vivo and in-vitro metabolism of tectoridin. After oral administration of a single dose (100 mg kg−1) of tectoridin to healthy rats, faeces and urine samples were collected for 0–48 h and 0–24 h, respectively. Tectoridin was also incubated with rat intestinal flora and rat liver microsomes. Samples from in-vivo and in-vitro metabolism studies were purified using a C18 solid-phase extraction cartridge, then separated using a reverse-phase C18 column with methanol/water (30:70, v/v, adjusted to pH 10.0 with ammonia water) as mobile phase and detected by an on-line MSn system. The structure of the metabolites was elucidated by comparing their molecular weights, retention times and full-scan MSn spectra with those of the parent drug. The results revealed six metabolites of tectoridin in urine (tectorigenin, hydrogenated tectorigenin, mono-hydroxylated tectorigenin, di-hydroxylated tectorigenin, glucuronide-conjugated tectorigenin and sulfate-conjugated tectorigenin); three metabolites in faeces (tectorigenin, di-hydroxylated tectorigenin and sulfate-conjugated tectorigenin); one metabolite in the intestinal flora incubation mixture (tectorigenin), and four in the liver microsomal incubation mixture (tectorigenin, hydrogenated tectorigenin, mono-hydroxylated tectorigenin and di-hydroxylated tectorigenin). Except for tectorigenin, all other metabolites of tectoridin are reported for the first time.