Detection of Pathogenic Escherichia coli (E. coli) Using Robust Silver and Gold Nanoparticles

Abstract

The paper deals with differently synthesized robust metal nanoparticles using chemical synthesis approach for detection of a new pathogenic strain E. coli in water sample. The reactions have been completed using aqueous metal salt solution and trisodium citrate as a capping agent to obtain nanoparticles. For different concentration of sodium borohydride, surface plasmon resonance peaks are obtained at 390 nm to 402 nm for silver nanoparticles and 518 nm to 524 nm for gold nanoparticles. It is to be shifted from 395 nm to 408 nm and 520 to 527 nm for gold and silver nanoparticles after the interaction with E. coli. These nanoparticles have also been synthesized at 80ºC, exhibited strong surface plasmon resonance peaks respectively at 433 nm and 529 nm. Remarkable shift can be noticed in surface plasma resonance peaks for small amount of E. coli added in the solution. The nanoparticles and their interaction against microorganisms were characterized using UV-Vis spectroscopy and Transmission Electron Microscopy (TEM).