Centrifugal Step Emulsification Microfluidics Supporting Droplet Digital Loop-Mediated Isothermal Amplification (LAMP) of Sars-Cov-2 N Gene

Abstract

Responding to the global crisis of SARS-CoV-2 (COVID-19), the centrifugal step emulsification microfluidics supporting droplet digital Loop-mediated isothermal amplification (LAMP) is presented to quantify the SARS-COV-2 N Gene in this study. The designed centrifugal step emulsification chip with a triangular nozzle shows a robust droplet generation for sample division for LAMP, and the influence by channel flow resistance is experimentally analyzed. In addition, an evaporation-resisted structure is applied to address the reagent loss in the heating process caused by PDMS material. The devices and solution are potential to be a promising tool for the quantification of SARS-CoV-2.