The effect of cinnamon to the expression of SOD-1 and TNF-α in indomethacin-induced gastric ulcer rat

Abstract

Gastric ulcer is a disease that commonly occurs and spreads over the world, and one of the most common causes is the use of long-term non-steroid anti-inflammatory drugs (NSAIDs). Active compounds of bark and leaves of the cinnamon extract are cinnamaldehyde and eugenol, which are acting as gastroprotective by antioxidant and anti-inflammatory properties and also flavonoids such as quercetin, kaempferol, and quercetrin as an antioxidant. This study aimed to determine the effect of cinnamon extract on the expression of SOD-1 and TNF-α in indomethacin-induced gastric ulcers. Indomethacin 30 mg/kg BW orally induced after 8 hour fasting period. Cinnamon extract administered in different doses daily for two days. The rats divided into s6 groups: nnegative control group, positive control group on first day, positive control group on third day, and three treatment groups (T1, T2, and T3) with cinnamon extract doses of 400, 200 and 100 mg/kg BW respectively. The level of SOD-1 measured by ELISA and the expression of TNF-α by immunohistochemistry from gastric mucosa. The results showed that cinnamon extract significantly increased SOD-1 and decrease of TNF-α. Histopathology analysis showed the improvement of gastric epithelial after cinnamon provision. These results demonstrate that the administration of cinnamon extract may protect indomethacin-induced gastric ulcer rats through their antioxidant and anti-inflammatory properties, and also it is improving gastric histopathology.