Changes in ribosomal ribonucleic acid content within in vitro-produced bovine embryos

Abstract

The abundance of 28S, 18S, and 5S rRNA was measured by Northern blot techniques applied to RNA samples extracted from bovine oocytes and preattachment embryos produced by in vitro procedures. Total RNA content was estimated by comparing the intensity of hybridization signals of 28S and 18S rRNA probes to embryo RNA samples and to standard curves generated from bovine ovary or bovine oviduct cell RNA. RNA content declined from the oocyte to the morula stage (2.4 ± 0.3 ng/oocyte, 1.7 ± 0.5 ng/1-cell embryo, 2.2 ± 0.9 ng/2- to 4-cell embryo, 0.8 ± 0.2 ng/6- to 8-cell embryo, and 0.7 ± 0.2 ng/morula). A marked increase in RNA content, based on levels of hybridization to 28S and 18S rRNA, was observed in blastocysts, in which values averaged 5.3 ± 0.6 ng/embryo. On a relative basis, 5S rRNA abundance followed a pattern similar to that of 28S and 18S rRNA across the early development period to the blastocyst stage.