Isolation of a cDNA encoding active protein for kiwifruit ACC synthase and its specific expression in the outer pericarp

Abstract

ACC (1-aminocyclopropane-1-carboxylate) synthase cDNA (AD-ACS1), isolated from kiwifruit 'Hayward', was expressed as a fusion protein coupled with glutathione S-transferase (GST) in Escherichia coli. The fusion protein exhibited ACC synthase activity. Northern blot analyses showed that the transcripts hybridized with AD-ACS1 accumulated only in the outer pericarp after a 24-hr ethylene treatment.