Isolation of intact high-molecular-weight DNA by using guanidine isothiocyanate.

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Abstract

A method for obtaining high-molecular-weight chromosomal DNA from Bacteroides intermedius and Bacteroides gingivalis is described. This technique is a modification of the guanidine isothiocyanate isolation procedure for RNA and should be useful for isolating intact DNA from organisms with high endogenous nuclease activity.

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Lippke, J. A., Strzempko, M. N., Raia, F. F., Simon, S. L., & French, C. K. (1987). Isolation of intact high-molecular-weight DNA by using guanidine isothiocyanate. Applied and Environmental Microbiology, 53(10), 2588–2589. https://doi.org/10.1128/aem.53.10.2588-2589.1987

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