Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean

Abstract

This manuscript reports on the application of microsatellite markers to assess the effectiveness of controlled crosses and assess the genetic identity of seed samples from a same common bean cultivar (BRS Estilo). The DNA was extracted from leaf tissue collected from F1 plants and their parents as well as from seed samples of the cv. BRS Estilo by the alkaline lysis method. In all cases, genotyping was carried out with 24 microsatellite markers distributed in four multiplex panels with six markers each. Of the 392 F1 plants analyzed, obtained from 21 different biparental crosses, 325 (82.91%) was confirmed as real hybrids by the molecular analysis. The genetic analysis of the four BRS Estilo seed samples (two of certified seeds and two of saved seeds) detected 100.00% genetic identity with the samples used as control in only one certified seed sample.