Development of polymorphic microsatellite markers for Incarvillea sinensis (Bignoniaceae)

Abstract

Premise of the study: Microsatellite markers were developed for Incarvillea sinensis var. sinensis (Bignoniaceae), an annual herb endemic to Inner Mongolia, to study the degree to which delayed self-fertilization is favored. Methods and Results: Eight polymorphic primer sets were isolated and characterized in two Inner Mongolia populations of I. sinensis var. sinensis with a relatively simple and fast subcloning method. Numbers of alleles per locus ranged from 2 to 7, with observed and expected heterozygosities ranging from 0 to 0.261 and from 0 to 0.778, respectively. Conclusions: These markers will be useful for future studies of self-fertilization adaptability in I. sinensis var. sinensis. © 2011 Botanical Society of America.