Enzymatic phosphatidylation of thiamin, pantothenic acid, and their derivatives

Abstract

Phospholipase D from Streptomyces sp. was found to catalyze the transfer reaction of the dipalmitoylphosphatidyl residue from 1,2-dipalmitoyl-3-sn- phosphatidylcholine to thiamin, pantothenic acid, and their derivatives in a biphasic system. The following phos-phatidylated compounds were synthesized: 1,2-dipalmitoyl-3-sn-phosphatidylthiamin, 1,2-dipalmitoyl-3-Sn- phosphatidylthiamin propyl disulfide, 1,2-dipalmitoyl-3-sn-phosphatidyl-thiamin tetrahydrofurfuryl disulfide, 1,2-dipalmitoyl-3-sn-phosphatidylpantothenic acid, and 1,2-dipalmitoyl-3-sn-phosphatidyl-pantothenyl ethyl ether.