Dissolution profile of partially purified bromelain from pineapple core encapsulated in alginate-guar gum with in vitro study of antiplatelet activity

Abstract

The aim of this study is to maintain the antiplatelet activity of pineapple core bromelain from degradation in gastric fluid by encapsulating the enzyme in alginate-guar gum crosslinked with glutaraldehyde. Bromelain isolation was followed by purification process such as ammonium sulfate precipitation and dialysis. The specific activity obtained showed an enhancement started for crude enzyme (20.11 U/mg), ammonium sulfate fraction (190.64 U/mg), and dialysis fraction (229.77 U/mg). Dialysis fraction was encapsulated by in situ loading method in alginate-guar gum hydrogel with 1.25% (v/v) glutaraldehyde concentration. The swelling ratio of the hydrogel is 234.37 % in acidity of 1.2; and 1375.91 % in acidity of 7.4; while the encapsulation efficiency amount was 60.42%. I n addition, maximum concentration of bromelain released in dissolution test was higher in artificial intestinal environment (1.79 mg/L) than in artificial gastric fluid (0.13 mg/L), with maximum proteolytic activity of 1.2 U/mL and 0.11 U/mL, respectively. In vitro study of antiplatelet activity showed a good inhibition for dialysis fraction (50.6 %) and dissolution fraction (34.86 %), indicating that the hydrogel is good enough to maintain the activity of bromelain.