The p85α Regulatory Subunit of Class IA Phosphoinositide 3-Kinase Regulates β-Selection in Thymocyte Development

Abstract

We examined the role of class IA PI3K in pre-TCR controlled β-selection and TCR-controlled positive/negative selection in thymic development. Using mice deficient for p85α, a major regulatory subunit of the class IA PI3K family, the role of class IA PI3K in β-selection was examined by injection of anti-CD3ε mAb into p85α−/−Rag-2−/− mice, which mimics pre-TCR signals. Transition of CD4−CD8− double-negative (DN) to CD4+CD8+ double-positive (DP) thymocytes triggered by anti-CD3ε mAb was significantly impaired in p85α−/−Rag-2−/− compared with p85α+/−Rag-2−/− mice. Furthermore, DP cell numbers were lower in p85α−/−DO11.10/Rag-2−/− TCR-transgenic mice than in DO11.10/Rag-2−/− mice. In addition, inhibition by IC87114 of the major class IA PI3K catalytic subunit expressed in lymphocytes, p110δ, blocked transition of DN to DP cells in embryonic day 14.5 fetal thymic organ culture without affecting cell viability. In the absence of phosphatase and tensin homolog deleted on chromosome 10, where class IA PI3K signals would be amplified, the DN to DP transition was accelerated. In contrast, neither positive nor negative selection in Rag-2−/−TCR-transgenic mice was perturbed by the lack of p85α. These findings establish an important function of class IA PI3K in the pre-TCR-controlled developmental transition of DN to DP thymocytes.