Lac repressor purification without inactivation of DNA binding activity

68Citations
Citations of this article
16Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

A procedure has been developed which eliminates the commonly observed inactivation of the DNA binding activity of the lac repressor during purification. The operator binding activity of the repressor obtained by this method is 100 ±10%. The repressor can be stored frozen indefinitely without losing its affinity for DNA. © 1977 Information Retrieval Limited.

Cite

CITATION STYLE

APA

Rosenberg, J. M., Khallai, O. B., Kopka, M. L., Dickerson, R. E., & Riggs, A. D. (1977). Lac repressor purification without inactivation of DNA binding activity. Nucleic Acids Research, 4(3), 567–572. https://doi.org/10.1093/nar/4.3.567

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free