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Lac repressor purification without inactivation of DNA binding activity

Nucleic Acids Research (1977) 4(3) 567-572
DOI: 10.1093/nar/4.3.567
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Abstract

A procedure has been developed which eliminates the commonly observed inactivation of the DNA binding activity of the lac repressor during purification. The operator binding activity of the repressor obtained by this method is 100 ±10%. The repressor can be stored frozen indefinitely without losing its affinity for DNA. © 1977 Information Retrieval Limited.

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Rosenberg, J. M., Khallai, O. B., Kopka, M. L., Dickerson, R. E., & Riggs, A. D. (1977). Lac repressor purification without inactivation of DNA binding activity. Nucleic Acids Research, 4(3), 567–572. https://doi.org/10.1093/nar/4.3.567

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