Molecular identification and phylogenetic status of marine Bacillus associated with coral sediment, showing antibacterial effects against human pathogens

Abstract

Ninety-three strains were collected from coral reef sediments and artificial mangrove forest in south India and screened for antibacterial property by cross-streaking assay against 11 human pathogens. Five different fractions of three positive strains (GM4, GMS and GMlO) were prepared and culture filtrate (CF), ethyl acetate extracts (EA) and intermediate opaque layer (IOL) fractions showed antibacterial activity against Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella enterica, Bacillus sp. and Proteus vulgaris. Genotyping using partial 16S rRNA gene and partial gyraseB sequencing revealed molecular identification (GM4-Bacillus cereus, GM5-Bacillus licheniformis and GM10-Bacillus subtilis), phylogenetic status and inter specific relationship among these three strains.