Intranasal Sendai virus-based SARS-CoV-2 vaccine using a mouse model

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Abstract

The coronavirus disease 2019 (COVID-19) epidemic remains worldwide. The usefulness of the intranasal vaccine and boost immunization against severe acute respiratory syndrome-related coronavirus (SARS-CoV-2) has recently received much attention. We developed an intranasal SARS-CoV-2 vaccine by loading the receptor binding domain of the S protein (S-RBD) of SARS-CoV-2 as an antigen into an F-deficient Sendai virus vector. After the S-RBD-Fd antigen with trimer formation ability was intranasally administered to mice, S-RBD-specific IgM, IgG, IgA, and neutralizing antibody titers were increased in serum or bronchoalveolar lavage fluid for 12 weeks. Furthermore, in mice that received a booster dose at week 8, a marked increase in neutralizing antibodies in the serum and bronchoalveolar lavage fluid was observed at the final evaluation at week 12, which neutralized the pseudotyped lentivirus expressing the SARS-CoV-2 spike protein, indicating the usefulness of the Sendai virus-based SARS-CoV-2 intranasal vaccine.

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APA

Morimoto, S., Saeki, K., Takeshita, M., Hirano, K., Shirakawa, M., Yamada, Y., … Okano, H. (2023). Intranasal Sendai virus-based SARS-CoV-2 vaccine using a mouse model. Genes to Cells, 28(1), 29–41. https://doi.org/10.1111/gtc.12992

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