Synthesis of [4S-2H]NADH, [4R-2H]NADH, [4-2H2]NADH and [4-2H]NAD+ cofactors through heterogeneous biocatalysis in heavy water

Abstract

This practitioner protocol describes the synthesis of a family of deuterated nicotinamide cofactors: [4S-2H]NADH, [4R-2H]NADH, [4-2H2]NADH and [4-2H]NAD+. The application of a recently developed H2-driven heterogeneous biocatalyst enables the cofactors to be prepared with high (>90%) 2H-incorporation with 2H2O as the only isotope source.