Diversidad genética de 12 loci microsatelitales utilizados en pruebas de paternidad equina en Chile

Abstract

The genetic diversity of equine microsatellite loci HMS2, HMS3, HMS6, HMS7, HTG4, HTG6, AHT4, AHT5, VHL20, ASB2, HTG7 and HTG10 was investigated in a population of Chilean Creole horses by means of parameters such as: the polymorphic information content, heterocigocity and excess or deficit of heterocigotes. In addition, the probability of paternity exclusion was considered. The results indicated that the genetic diversity of these markers was significantly high in the studied population. All markers analyzed were polymorphic. The number of alleles per locus, varied between 4 (HMS10) to 10 (HTG10). The expected average heterocigocity (He) considering all loci simultaneously, was 0.76 ranging from 0.52 to 0.88. The average observed heterocigocity (Ho) for all the markers was 0.65. The paternity exclusion probability per locus, oscillated between 14 and 59%. The power paternity exclusion considering the 12 loci was 99,7%.