Isolation and characterization of commercial probiotics

Abstract

Commercial probiotics have bacteria which offer scope for specific treatment as a probiotic against diarrhoea, boosting immune response and relieving stress. Research in probiotics and aquaculture has been done to promote sustainable aquaculture. Studies on microbial ecology in aquaculture and their benefits on human systems are to be assessed. Hence, the present study was aimed at the isolation and characterization of these bacteria obtained from various commercial probiotics. The commercial probiotic samples were collected for isolation using MRS agar. The colonies were selected on the basis of colonial morphology. Isolates were put through cell morphology, physiology and different biochemical tests. Probiotic sample confirmation was done using 16srRNA molecular technique. MEGA7 was used to conduct phylogenetic evolutionary analysis and tabulate a distance matrix. In results, the isolates manifested non-identical growth patterns at dissimilar conc. of NaCl (2.0,4.0 and 6.5) oxygen and at different temperatures (150 C, 300 C and 450 C). On the basis of sugar utilization, physiological testing, biochemical characterization and genetic identification tests, all isolates were established to different species of Lactobacillus rhamnosus, Pediococcus acidilactici and Lactobacillus Plantarum. A systematic protocol was done to identify, characterize probiotic samples and identify them by genetic analysis. Probiotic use is carefully assessed by regulations.