Microbial parameters as indicators of mariculture impact on the marine environment

Abstract

Microbial abundance and extracellular activity rates (leucine aminopeptidase, LAP, beta-glucosidase, β-GLU, and alkaline phosphatase, AP) were studied in water and sediment samples for two years in three Sicilian mariculture sites. Microbial activities were measured with specific fluorogenic substrates; heterotrophic culturable bacteria (CFU) were counted on Marine agar plates. The amount of particulate organic matter (POM) and its composition in terms of bio-polymeric fractions (proteins, carbohydrates, lipids) were also monitored as indicators of trophic conditions. Cage sites were sampled before and after the set up of each off-shore mariculture plant, and compared to "control" sites. The study aimed at monitoring the impact caused by fish farming activities on the marine environment and at assessing the suitability of these microbial parameters as indirect indicators of organic pollution. Significant variations occurred both in microbial activity rates and in abundances at cage stations. Compared with the trophic parameters, the microbial parameters showed a more direct response to environmental perturbation due to fish farming. This suggested their use as indicators in assessing fish farming impact on both the pelagic and benthic compartments. © 2010 WIT Press.