Monitoring BCR-ABL transcript levels by real-time quantitative polymerase chain reaction: A linear regression equation to convert from BCR-ABL/B2M ratio to estimated BCR-ABL/ABL ratio

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Abstract

In order to overcome the problem of different control genes for BCR-ABL normalization, we used a linear regression equation to compare our results previously obtained using B2M as the control gene with those calculated using the ABL gene and validated the slope as a factor to convert from B2M to ABL results.

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Iacobucci, I., Galletti, L., Amabile, M., Soverini, S., Baccarani, M., & Martinelli, G. (2007). Monitoring BCR-ABL transcript levels by real-time quantitative polymerase chain reaction: A linear regression equation to convert from BCR-ABL/B2M ratio to estimated BCR-ABL/ABL ratio. Haematologica, 92(3), 429–430. https://doi.org/10.3324/haematol.10632

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