Bulk pollen pollination in maize for efficient construction of introgression populations with high genome coverage

Abstract

Introgression populations consist of a set of introgression lines or families, constructed by continuous backcrossing to the recurrent parent, while carrying a limited number of chromosome segments from a donor parent in their genomes. Increasing the genome coverage is an important aim when constructing introgression population. In this study, we proposed bulk pollen pollination (BPP) method and used it to increase the genome coverage of a maize introgression population. The results showed that the genome coverage of the introgression population constructed using BPP method reached 100% at BC3 generation, which accorded with the simulation result. The BPP-based BC3F1:2 population could identify most quantitative trait loci (QTL) detected using the F2:3 population, especially major QTL. Simulation analysis showed that the genome coverage of introgression population increased with the increase of population size and the number of bulked plants, and decreased with the increase of backcross generation. Our results proved the reliability of the BPP-based introgression population in increasing genome coverage and detecting QTL, and provided references for constructing high-coverage introgression populations.