Potential Test Development of Dengue Hemorrhagic Fever Medicine from Jatropha Multifida Stem Bark as Organic Chemistry Teaching Material

Abstract

Jatropha multifida L. is one of the medicinal plants that Bengkulunese use as a new wound medicine inherited by the ancestors. The purpose of this research is to test J. multifida as a medicine of DHF through preclinical trials in mice. Furthermore, this laboratory research was developed as a teaching material, with the ADDIE model. The research started with phytochemical test of bark extract. 40 mice were divided into eight groups; each of which consisted of 5 mice. The group of P0 mice were given olive oil using gavage, group P1 was given aspirin orally, group P2, P3 and P4 mice were given crude extract of J. multifida with consecutive dosage of 0.028 g/kgbw; 0.056 g/kgbw and 0.084g/kgbw. The groups P5, P6 and P7 were given ethyl acetate extract at the dosage of 0.028 g/kgbw; 0.056 g/kgbw and 0.084g/kgbw. The steps of the study were started from the phytochemical test. The treatment of each group of mice and the results of treatment after analysis, design, development, implementation, evaluation were then developed as teaching materials. Crude and ethyl acetate extracted from J. multifida barks can increase the platelet count of mice suffering from thrombocytopenia. The administration of crude extracts and ethyl acetate extract from J. multifida stem barks at the dose of 0.028 g/kgbw; 0.056 g/kgbw and 0.84g/kgbw can increase the platelet count of mice with trombocytopenia. Particularly in the administration of crude J. multifida at a dose of 0.028 g/kgbw can increase the number of platelet up to normal in thrompbocytopenic mice. Based on that fact, crude extracts from J. multifida barks are highly potential to be used as a medicine to increase number of platelet in DHF patients. Teaching materials developed from this case, the test activity of crude extract and ethyl acetate extract from J. multifida stem bark to increase the number of platelets, provide new knowledge for the students and engage them in active learning proven by the response of the students that are in very good categories.